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Figure 3

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ZDB-IMAGE-210503-17
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Figures for Kamel et al., 2021
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Figure 3 Hearts of adult tnnt2a+/RK94del display increased myocardial stress and fibrosis. (A–C) In situ hybridization on 4-month-old adult heart sections of tnnt2a+/+, tnnt2a+/RK94del, and tnnt2a+/hu11260 stained for nppb, a marker of myocardial stress. In situ hybridization on adult heart sections of tnnt2a+/+ and tnnt2a+/RK94del stained for (D,E) tnnt2a, to mark cardiac Troponin T expression, and (F,G) col6a1, which marks collagen. (H,I) AFOG staining, which marks collagen in blue, muscle in orange, and fibrin in red of tnnt2a+/+ and tnnt2a+/RK94del. (J,K) In situ hybridization on adult heart sections of tnnt2a+/+ and tnnt2a+/RK94del stained for postnb, to mark fibroblasts. Images were taken at a magnification of 20×, except for (H,I) where magnification was at 60×. Scale bars are 200 µm for whole-heart tile scans, 50 µm for zoom-in regions, and 20 µm for zoom-in regions of H, I. A: atrium, V: ventricle, BA: bulbus arteriosus.

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This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J Cardiovasc Dev Dis