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Fig. 1 ctage5 and tango1 mutations affect size and survival of zebrafish: (A) Schematic showing CRISPR guide RNA cut sites (scissors) and the estimated final truncated proteins. (B) Representative brightfield images of all combinations of ctage5 and tango1 mutations in 4dpf zebrafish larvae. Y = yolk, SB = swim bladder, E = eye. (C) Length (one-way ANOVA, F = 38.6, p < 0.0001), (D) mass (one-way ANOVA, F = 28.22, p < 0.0001) and (E) survival (χ2 = 24.59, p < 0.05, n = 59) measurements of all combinations of ctage5 and tango1 mutant zebrafish at 2 mo raised in a low-density environment (about 10 fish per tank). Red-dotted lines in E represent expected survival, and black bars are actual survival. ** = p < 0.01, *** = p < 0.001, **** = p < 0.0001.