PUBLICATION

Complementary and divergent functions of zebrafish Tango1 and Ctage5 in tissue development and homeostasis

Authors

Clark, E.M., Link, B.A.

ID

ZDB-PUB-210114-16

Date

2021

Source

Molecular biology of the cell 32(5): 391-401 (Journal)

Registered Authors

Link, Brian

Keywords

none

MeSH Terms

Carrier Proteins/metabolism
Protein Transport/physiology
Antigens, Neoplasm/genetics
Antigens, Neoplasm/metabolism
COP-Coated Vesicles/metabolism
Golgi Apparatus/metabolism
Zebrafish/genetics
Zebrafish/metabolism
Endoplasmic Reticulum/metabolism
Homeostasis
Animals
Vesicular Transport Proteins/metabolism
Zebrafish Proteins/genetics
Zebrafish Proteins/metabolism
Aryl Hydrocarbon Receptor Nuclear Translocator/genetics*
Aryl Hydrocarbon Receptor Nuclear Translocator/metabolism
Neoplasm Proteins/genetics*
Neoplasm Proteins/metabolism

(all 18)

PubMed

33439675 Full text @ Mol. Biol. Cell

Abstract

Coat protein complex II (COPII) factors mediate cargo export from the endoplasmic reticulum (ER), but bulky collagens and lipoproteins are too large for traditional COPII vesicles. Mammalian CTAGE5 and TANGO1 have been well characterized individually as specialized cargo receptors at the ER that function with COPII coats to facilitate trafficking of bulky cargoes. Here, we present a genetic interaction study in zebrafish of deletions in ctage5, tango1, or both to investigate their potential distinct and complementary functions. We found that Ctage5 and Tango1 have different roles related to organogenesis, collagen versus lipoprotein trafficking, stress-pathway activation, and survival. While disruption of both ctage5 and tango1 compounded phenotype severity, mutation of either factor alone revealed novel tissue specific defects in the building of heart, muscle, lens, and intestine, in addition to the previously described roles in the development of neural and cartilage tissues. Together, our results demonstrate that Ctage5 and Tango1 have overlapping functions, but also suggest divergent roles in tissue development and homeostasis.

Genes / Markers

Marker	Marker Type	Name
mia2	GENE	MIA SH3 domain ER export factor 2
mia3	GENE	MIA SH3 domain ER export factor 3

Figures

Show all Figures

Expression

Gene	Fish	Conditions	Stage	Anatomy	Assay	Figure
d2EGFP	mia2^mw91/mw91; mia3^mw92/mw92; mw85Tg	standard conditions	Day 4	spinal cord	IFL	Fig. 4
d2EGFP	mia2^mw91/mw91; mia3^mw92/mw92; mw85Tg	standard conditions	Day 4	trunk muscle	IFL	Fig. 5
d2EGFP	mia2^mw91/mw91; mia3^mw92/mw92; mw85Tg	standard conditions	Day 4	ventral mandibular arch	IFL	Fig. 5
d2EGFP	mia2^mw91/mw91; mw85Tg	standard conditions	Days 7-13	intestine	IFL	Fig. 5
d2EGFP	mia2^mw91/mw91; mw85Tg	standard conditions	Days 7-13	spinal cord	IFL	Fig. 4
d2EGFP	mia2^mw91/mw91; mw85Tg	standard conditions	Day 4	spinal cord	IFL	Fig. 4
d2EGFP	mia2^mw91/mw91; mw85Tg	standard conditions	Days 7-13	trunk muscle	IFL	Fig. 5
d2EGFP	mia2^mw91/mw91; mw85Tg	standard conditions	Day 4	trunk muscle	IFL	Fig. 5
d2EGFP	mia2^mw91/mw91; mw85Tg	standard conditions	Days 7-13	ventral mandibular arch	IFL	Fig. 5
d2EGFP	mia2^mw91/mw91; mw85Tg	standard conditions	Day 4	ventral mandibular arch	IFL	Fig. 5

1 - 10 of 42

Show

Phenotype

Fish	Conditions	Stage	Phenotype	Figure
mia2^mw91/+	standard conditions	Day 4	whole organism morphology, normal	Fig. 1
mia2^mw91/+	standard conditions	Days 45-89	whole organism normal length, normal	Fig. 1
mia2^mw91/+	standard conditions	Days 45-89	whole organism weight, normal	Fig. 1
mia2^mw91/+; mia3^mw92/+	standard conditions	Day 4	whole organism morphology, normal	Fig. 1
mia2^mw91/+; mia3^mw92/+	standard conditions	Days 45-89	whole organism normal length, normal	Fig. 1
mia2^mw91/+; mia3^mw92/+	standard conditions	Days 45-89	whole organism weight, normal	Fig. 1
mia2^mw91/+; mia3^mw92/mw92	standard conditions	Day 4	head deformed, abnormal	Fig. 1
mia2^mw91/+; mia3^mw92/mw92	standard conditions	Day 4	mouth decreased length, abnormal	Fig. 1
mia2^mw91/+; mia3^mw92/mw92	standard conditions	Days 45-89	whole organism dead, abnormal	Fig. 1
mia2^mw91/+; mia3^mw92/mw92	standard conditions	Days 45-89	whole organism decreased life span, abnormal	Fig. 1

1 - 10 of 74

Show

Mutations / Transgenics

Allele	Construct	Type	Affected Genomic Region
mil1Tg	Tg(Tru.Gap43:EGFP)	Transgenic Insertion
mw85Tg	Tg(5xHsa.ATF6-fosab:d2EGFP)	Transgenic Insertion
mw91		Small Deletion	mia2
mw92		Small Deletion	mia3

1 - 4 of 4

Show

Human Disease / Model

Sequence Targeting Reagents

Target	Reagent	Reagent Type
mia2	CRISPR1-mia2	CRISPR
mia2	CRISPR2-mia2	CRISPR
mia3	CRISPR1-mia3	CRISPR
mia3	CRISPR2-mia3	CRISPR

Fish

Fish
mia2^mw91/mw91
mia2^mw91/mw91; mia3^mw92/mw92
mia2^mw91/mw91; mia3^mw92/mw92; mw85Tg
mia2^mw91/mw91; mia3^mw92/+
mia2^mw91/mw91; mil1Tg
mia2^mw91/mw91; mw85Tg
mia2^mw91/+
mia2^mw91/+; mia3^mw92/mw92
mia2^mw91/+; mia3^mw92/+
mia3^mw92/mw92

1 - 10 of 15

Show

Antibodies

Name	Type	Antigen Genes	Isotypes	Host Organism
Ab1-col2a	monoclonal		IgG1	Mouse

Orthology

Engineered Foreign Genes

Marker	Marker Type	Name
d2EGFP	EFG	d2EGFP
EGFP	EFG	EGFP
GAL4	EFG	GAL4
mCherry	EFG	mCherry

Mapping

Clark et al., 2021 ZDB-PUB-210114-16 PMID:33439675

Complementary and divergent functions of zebrafish Tango1 and Ctage5 in tissue development and homeostasis

Clark et al., 2021

ZDB-PUB-210114-16
PMID:33439675