PUBLICATION

Complementary and divergent functions of zebrafish Tango1 and Ctage5 in tissue development and homeostasis

Authors
Clark, E.M., Link, B.A.
ID
ZDB-PUB-210114-16
Date
2021
Source
Molecular biology of the cell   32(5): 391-401 (Journal)
Registered Authors
Link, Brian
Keywords
none
MeSH Terms
  • Carrier Proteins/metabolism
  • Protein Transport/physiology
  • Antigens, Neoplasm/genetics
  • Antigens, Neoplasm/metabolism
  • COP-Coated Vesicles/metabolism
  • Golgi Apparatus/metabolism
  • Zebrafish/genetics
  • Zebrafish/metabolism
  • Endoplasmic Reticulum/metabolism
  • Homeostasis
  • Animals
  • Vesicular Transport Proteins/metabolism
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism
  • Aryl Hydrocarbon Receptor Nuclear Translocator/genetics*
  • Aryl Hydrocarbon Receptor Nuclear Translocator/metabolism
  • Neoplasm Proteins/genetics*
  • Neoplasm Proteins/metabolism
(all 18)
PubMed
33439675 Full text @ Mol. Biol. Cell
Abstract
Coat protein complex II (COPII) factors mediate cargo export from the endoplasmic reticulum (ER), but bulky collagens and lipoproteins are too large for traditional COPII vesicles. Mammalian CTAGE5 and TANGO1 have been well characterized individually as specialized cargo receptors at the ER that function with COPII coats to facilitate trafficking of bulky cargoes. Here, we present a genetic interaction study in zebrafish of deletions in ctage5, tango1, or both to investigate their potential distinct and complementary functions. We found that Ctage5 and Tango1 have different roles related to organogenesis, collagen versus lipoprotein trafficking, stress-pathway activation, and survival. While disruption of both ctage5 and tango1 compounded phenotype severity, mutation of either factor alone revealed novel tissue specific defects in the building of heart, muscle, lens, and intestine, in addition to the previously described roles in the development of neural and cartilage tissues. Together, our results demonstrate that Ctage5 and Tango1 have overlapping functions, but also suggest divergent roles in tissue development and homeostasis.
Genes / Markers
Marker Marker Type Name
mia2GENEMIA SH3 domain ER export factor 2
mia3GENEMIA SH3 domain ER export factor 3
1 - 2 of 2
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Figures
Figure Gallery (6 images)
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Expression
Phenotype
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
mil1TgTransgenic Insertion
    mw85TgTransgenic Insertion
      mw91
        Small Deletion
        mw92
          Small Deletion
          1 - 4 of 4
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          Human Disease / Model
          No data available
          Sequence Targeting Reagents
          Target Reagent Reagent Type
          mia2CRISPR1-mia2CRISPR
          mia2CRISPR2-mia2CRISPR
          mia3CRISPR1-mia3CRISPR
          mia3CRISPR2-mia3CRISPR
          1 - 4 of 4
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          Fish
          Antibodies
          Name Type Antigen Genes Isotypes Host Organism
          Ab1-col2amonoclonal
            IgG1Mouse
            1 - 1 of 1
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            Orthology
            No data available
            Engineered Foreign Genes
            Marker Marker Type Name
            d2EGFPEFGd2EGFP
            EGFPEFGEGFP
            GAL4EFGGAL4
            mCherryEFGmCherry
            1 - 4 of 4
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            Mapping
            No data available