Figure 8.

Figure 8.

Time-lapse imaging of myelin sheath formation in zebrafish exposed to domoic acid (DomA) at 2 d postfertilization (dpf). (A) Tg(mbp:EGFP-CAAX) fish were used to visualize labeled myelin sheaths. Shown are representative images of DomA-exposed (0.14 ng) (right panel), and control larvae (left panel) that were exposed at 2 dpf and imaged at 3 dpf. White arrowheads denote the aberrant circular protrusions found in DomA-exposed larvae (control, n=5; DomA, n=10). (B) Stills from time-lapse imaging of a representative control and DomA-exposed transgenic fish [Tg(nkx2.2:mEGFP)×Tg(sox10:RFP)] from 2.5–3 dpf. Time stamps (hour:minutes) show the time elapsed. Diagrams above the images show the key developmental processes in the oligodendrocyte lineage during this time range (representative time-lapse image of control, n=5 and DomA, n=6). Large yellow arrowhead denotes an elongated myelin sheath. Small white arrowheads denote unusual circular myelin membranes. Scale bar: 100μm. Stills (B) were from a time lapse of control (Video S2) and DomA-exposed fish (Video S3).