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FIGURE 5

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ZDB-IMAGE-200423-21
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Figures for Carter et al., 2020
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FIGURE 5

eGFP-Rab28 localization in 1 mpf zebrafish cone photoreceptors. (A–C) Representative confocal z-projections of eGFP-Rab28 localization in 1 mpf zebrafish cones. Cone OS are stained with anti-cone transducin alpha antibody. Each tier of photoreceptors is comprised of different classes of cone. DC: double cones; LSC: long single cones; SSC: short single cones. Scale bars 10 μm. For WT, Q72L and T26N eGFP-Rab28 reporters a total of 13, 11 and 11 retinas across at least six individuals were imaged, respectively. (D–F) Deconvolved, high resolution confocal z-projections of eGFP-Rab28 WT, Q72L and T26N mutant localization in 1 mpf cones. Arrowheads point to discrete bands present throughout the OS. Scale bars 5 μm.

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