Figure 2

Figure 2

(A) Genotyping results identifying homozygote (dlx3b^−∕−) mutant and wild-type siblings (dlx3b^+∕+). Genomic DNA was amplified by PCR from the tails of the first-generation (F1) fish and then digested with a diagnostic restriction enzyme (Hind III). The first lane contains the original PCR product (393 bp). Lanes 2 and 3 contain wild-type siblings (dlx3b^+∕+), and lanes 4 and 5 contain homozygotes (dlx3b^−∕−). (B) Sequencing peak map of the DNA sample from the wild-type siblings (dlx3b^+∕+) and mutant (dlx3b^−∕−) zebrafish, respectively. The absence of the four-bp sequence (AAGC) in the mutant zebrafish (dlx3b^−∕−) is clearly marked. (C) Homozygous mutant (dlx3b^−∕−) zebrafish exhibited a lower survival rate compared with that of their wild-type (dlx3b^+∕+) and heterozygous siblings (dlx3b^+∕−) from 6–10 dpf. Within 5 d (6–9 dpf), the survival rate of the dlx3b^−∕− group decreased from 100% to approximately 35%, while those of the dlx3b^+∕+ group and dlx3b^+∕− group decreased to 75%–80%.^∗P < 0.05 vs. The dlx3b^+∕+ group. n = 100 for each group (the starting population).