IMAGE

Figure 4

ID
ZDB-IMAGE-200102-6
Antibodies
Source
Figures for Tonelotto et al., 2019
Image
Figure Caption

Figure 4

Spatio-temporal pattern of ColVI distribution in larvae from wild-type animals and transgenic reporter fish lines. (a-a’) Individual z-stack of whole-mount immunofluorescence for ColVI (red) and collagen II (green) in 3-dpf larvae. Ventral view. A strong ColVI labeling in evident in the connective tissue surrounding craniofacial cartilages and in the jaw joint. Panel a’ is a magnification of the dotted area of panel a, showing the deposition of ColVI in the jaw joint. (b-b’) Confocal z-stacks of whole-mount immunofluorescence for ColVI (red) in 6-dpf Tg(7xTCF-Xla.Siam:GFP)ia4 (TCF-GFP, green) larvae, showing ColVI labeling near Wnt-positive cells in craniofacial cartilages. Ventral view. Panel b’ is a magnification of the dotted area of panel b, showing ColVI labeling in close apposition to Wnt-positive cells in the ceratohyal cartilages. (c-f’) Section of 7-dpf larvae stained with anti-α1(VI) antibodies (red), WGA (green) and anti-MF20 antibodies (gray). ColVI labeling is present in craniofacial cartilaginous elements, as well as in blood vessels (arrowhead). Panel f’ is a magnification of the dotted area of panel f, showing ColVI labeling in the perichondrium surrounding the cartilaginous elements of the pharyngeal arches, as revealed by co-localization with WGA. (gk) Individual z-stack of whole mount immunostaining of 6-dpf Tg(osx:nuGFP) (osx:GFP, green) larvae operculum, stained with ColVI (red) and collagen XII (gray) antibodies and Hoechst (blue). ColVI and collagen XII surround operculum osteoblasts. (ln) Section of a 7-dpf larva stained with anti-ColVI antibodies (red), WGA (green) and Hoechst (blue). ColVI labeling is present in the connective tissue around brain, olfactory pit and eye. (o) Section of a 7-dpf larva stained with anti-ColVI antibodies (red) and Hoechst (blue), showing ColVI labeling in the meninges (arrowhead). (p) Confocal z-stacks of whole-mount immunofluorescence for ColVI (red) and collagen XII (green) on 6 dpf larvae eye, showing ColVI deposition in the connective tissue surrounding the eye. Dorsal view. (qu) Individual z-stack of whole mount immunostaining for ColVI (red), collagen XII (gray) antibodies and Hoechst (blue) in 2-dpf Tg(fli1:EGFP) (fli:EGFP, green) larvae at the level of pharyngeal arches. ColVI and collagen XII are part of the bordering tissue of migrating pharyngeal pouches. (vx) Confocal z-stacks of whole-mount immunofluorescence for ColVI (red) in 6-dpf Tg(fli1:EGFP) (fli:EGFP, green) larvae. A strong pattern of ColVI labeling is found around blood vessels (arrowheads) of the intestine. (y-z) Confocal z-stacks of whole-mount immunofluorescence for ColVI (red) and collagen XII (green) in 6-dpf larvae, showing that ColVI but not collagen XII is deposited in the intestine. Scale bar, 100 µm (vx) or 50 µm (au,y,z). cb, ceratobranchial; ch, ceratohyal; ey, eye; f, forebrain; h, hindbrain; jj, jaw joint; mc, Meckel’s cartilage; mid, midbrain; op, olfactory pit; ope, operculum; pa, pharyngeal arch; pq, palatoquadrate.

Figure Data
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Sci. Rep.