IMAGE

Fig. 5.

ID
ZDB-IMAGE-191230-518
Genes
Source
Figures for Rougeot et al., 2019
Image
Figure Caption

Fig. 5.

Transcription factor expression is spatially dysregulated in MZezh2 mutant (MZezh2−/−) embryos. (A-C) Spatial expression analysis by (A) in situ hybridization, (B) RT-qPCR on anterior half and posterior half, and (C) RNA-seq results of transcription factors tbx2a, tbx2b, tbx3a, tbx5a, isl1 and gsc in 24 hpf embryos. Scale bars: 1 mm. Experiments were performed in biological duplicates of a least 15 pooled embryos for in situ hybridization and in triplicates or quadruplicates of 20 pooled larval halves for RT-qPCR. Relative expression was calculated based on expression of the housekeeping gene actb1. Data are mean±s.e.m. in B with dots representing individual RT-qPCR samples. Relative expression was compared between anterior (red) or posterior (turquoise) parts in MZezh2−/− and wild-type embryos (one-way ANOVA with post-hoc tests, ***P<0.001, **P<0.01, *P<0.05). (C) Box plots represent normalized counts from RNA-seq experiments in whole MZezh2−/− and wild type after differential expression analysis with DEseq2. all, anterior lateral lane ganglion; ba, branchial arch; cmn, cranial motor neurons; de, diencephalon; drp, distal region of the pronephros; dscn, dorsal spinal cord neurons; e, eye; ep, epiphysis; fn, forebrain nuclei; h, heart; hmn, hindbrain motor neurons; llg, lateral lane ganglion; mot, primary motor neurons; og, olfactory ganglion; ov, otic vesicle; pa, pharyngeal arches; pan, pancreas; pf, pectoral fin; pro, pronephros; sc, spinal cord; tdn, telencephalon and diencephalon nuclei; te, telencephalon; vg, ventral ganglion. The box represents the first quartile, median and third quartile. The whiskers below and above the box represent the minimum and maximum values.

Figure Data
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development