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Fig. 5
HAB labels specific cells in live zebrafish from 32 hpf. Confocal fluorescence imaging of HAB labeling (10 μM) in live wild-type zebrafish embryos (32 and 48 hpf) and swimming larvae (72 hpf) following excitation at 488 nm and detection in the 550–650 nm range. The yellow-orange color is indicative of the fluorescence seen with the naked eye. Maximum intensity Z-projection images (2 μm serial optical sections) are shown. Arrows point to the HAB label; asterisks mark pigment cells. A, artery; N, notochord; V, vein.
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