ZFIN Image: Zhang et al., 2017, Fig. 3

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Figure Caption

Fig. 3

Comparison of the mCherry-MBD and 5 mC distribution patterns. (a) The distribution patterns of mCherry-MBD and anti-5 mC staining at 5 hpf. Higher magnification images are arranged below. White arrowheads label compacted puncta of chromatin. (b) The distribution patterns of mCherry-MBD and 5 mC at 24 hpf and 48 hpf. White boxes below are magnified 24 hpf images. (c) Colocalization analysis between mCherry-MBD, 5 mC, and heterochromatin (N = 12). (d) Comparison of nuclei from control embryos, and embryos treated by 5-dAZA or injected with dnmt1 MO. White boxes at right bottom are magnifications of each enclosed cell. (e) Fluorescence intensity of cells in each group (N = 16). The intensity value ranged from 0 to 255. P < 0.05. The intensity spectrums represented by the standard deviation are listed below.

Acknowledgments

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