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Fig. 3

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ZDB-IMAGE-171006-24
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Figures for Boer et al., 2016
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Fig. 3

Late-stage NC-cell migration is disrupted in fscn1a-morphant embryos.

(A-B) Dorsal cranial views of tp53zdf1 embryos injected with coMO or fscn1aMO and analyzed by whole-mount in situ hybridization (ISH) for (A) foxd3 mRNA at 10 hpf and (B) sox10 mRNA at 15 hpf. (C) Dorsal cranial and lateral views of 26 hpf tp53zdf1 embryos injected with coMO or fscn1aMO and analyzed by whole-mount ISH for dlx2a. Numbers correspond to pharyngeal arches. Arrow denotes reduction in dlx2a-positive cranial NC cells in fscn1a morphants. (D) Lateral views of cranial NC streams in 22, 25, 28 and 36 hpf Tg(sox10:gfp) embryos injected with tp53MO or tp53MO plus fscn1aMO. Numbers correspond to NC streams. Arrows highlight NC cells migrating independently of NC streams in fscn1a morphants. e; eye, ov; otic vesicle. In all panels, anterior is to the left. All experiments in this figure were performed independently at least three times with similar results. All scale bars in this figure = 100 μm.

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