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Fig. S4
Basal laminae are intact in the col4a5 mutant, related to Figures 4 and 5.
(A) representative ultrastructure of wild type neuromuscular junction (n = 3 larvae, 23 NMJs).
(B) higher magnification showing details of wild type junctional gap.
(C) col4a5 neuromuscular junction (n = 3 larvae, 17 NMJs).
(D) higher magnification showing details of col4a5 junctional gap. Green shading, axon; magenta arrowheads, plasma membranes enclosing basement membrane; m, muscle.
(E) Representative cross-section of wild type dorsal nerve axons (green) exiting the spinal cord surrounded by glia (red) and basal lamina (cyan) (n = 10 larvae, 30 nerves).
(F) Oblique optical cross section from (E) showing detail of dorsal axon exit and choice point.
(G) Cross-section of col4a5 dorsal nerve axons exiting with glia and basal lamina (n = 10 larvae, 30 nerves). (H) Oblique optical cross section from (G).