ZFIN Image: Isaacman-Beck et al., 2015, Fig. 1

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Figure Caption

Fig. 1

Regenerating Motor Axons Select Their Original Trajectory with High Fidelity

(A and B) A schematic (A) and in vivo imaging (B) demonstrate that zebrafish peripheral motor axons traverse a common path then diverge to innervate functionally distinct myotomal regions (dashed triangle, dorsal ROI; dashed boxes, nerve transection site; scale bar, 10 µm).

(C) By 48 hpt, dorsal axons regrow with great fidelity to the original trajectory (80% of fascicles that developed in the dorsal ROI regrew on the dorsal path; n = 14 larvae, 26 nerves; red arrowhead, misguided regrowth).

In (B) and (C), we omitted Tg(Xla.Tubb:DsRed) signal from the dorsal panel as expression in the spinal cord overwhelms the max projection image. The ventral panel shows expression from both transgenes.

Acknowledgments

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