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Fig. 3
Assessment of hepatic fibrosis in overfed zebrafish. (A) Sirius Red staining of liver sections from young males, old males, young females and old female zebrafish at baseline, and after 24weeks of overfeeding. Scale bar: 30µm. (B) Quantification of liver fibrosis by using computer-assisted digital image analysis. Data are reported as collagen proportionate area (CPA) converted into a percentage. Three sections from five fish per subgroup were analyzed. Values are expressed as means±s.d. *P<0.0001 for young females versus all the other groups.
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