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Fig. 7
Knockdown of sox2 results in abnormal parapineal development and disruption of the habenular asymmetries.
(A) gfi1ab is expressed in parapineal cells on the left side of the brain in control embryos. (B–D) sox2 morphants are categorized into three groups according to gfi1ab expression: left-sided expression (B), right-sided (C) and embryos with scattered gfi1ab cells (D). (E) kctd12.1 is asymmetrically expressed in the habenulae, with a broader expression domain in the left than the right habenula. (F–H) In sox2 morphants kctd12.1 expression can be: asymmetric with more on the left side similar to control embryos (F), asymmetric with more on the right side (G) or symmetric (H). (I) A table showing the percentage of embryos with normal, reversed or bilateral parapineal organs, using different staining methods. Scale bars = 25 μm. See also Figure S9–S10 and Movie S4.