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Fig. 5

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ZDB-IMAGE-120627-7
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Figures for Schneider et al., 2012
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Fig. 5 Expression profile of the zebrafish 5-HT2C receptor gene in different tissues generated by RT-PCR. A 310 bp fragment of the gene can be amplified from cDNA of zebrafish brain, skin, ovary, and axial muscle. The specific primers (KMXfwd/KMXrev) flank intron 4. Other tissues have not been tested. In addition, the receptor is expressed in 8 hpf and 2.5 hpf embryos. For the negative (neg) control, no cDNA was used for amplification. For the positive (pos) control, a plasmid containing the entire coding region of the receptor was used instead of cDNA. No quantitative approach was taken and the general intensity of individual fragments is not a direct indication of expression levels in individual tissues.

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Reprinted from Gene, 502(2), Schneider, H., Fritzky, L., Williams, J., Heumann, C., Yochum, M., Pattar, K., Noppert, G., Mock, V., and Hawley, E., Cloning and expression of a zebrafish 5-HT(2C) receptor gene, 108-117, Copyright (2012) with permission from Elsevier. Full text @ Gene