ZFIN Image: Shao et al., 2012, Fig. 2

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Figure Caption

Fig. 2

0-mpf lithium treatment activates Wnt/β-catenin signaling at mid-blastula stage and expands the organizer region.

(A and B) Confocal immunofluoresence image of of β-catenin in a 0-mpf NaCl treated embryo (A) and a 0-mpf lithium treated embryo (B) To ensure that the entire marginal zone is investigated, each embryo was scanned for two focal planes near the marginal zone of two hemispheres. The embryos were at sphere stage. Nuclear β-catenin was marked with white asterisks. (C–E) Expression pattern of organizer gene gsc in untreated (C), 0-mpf lithium treated (D), and 2-cell-stage lithium treated (E) embryos. All the embryos were animal pole view, dorsal up if it can be distinguished.

Acknowledgments

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