IMAGE

Fig. S3

ID
ZDB-IMAGE-110909-20
Source
Figures for Poulain et al., 2011
Image
Figure Caption

Fig. S3

Excess wnt2 or wnt2bb promotes hepatoblast specification and proliferation. (A) The wnt2bb overexpression construct. The hsp70 promoter controls expression of a mCherry-2A-wnt2bb transcript, which upon translation produces independent mCherry and Wnt2bb protein, owing to an unstable bond in the viral 2A-linker peptide. (B) The phenotypic rescue in a subset of prt/wnt2bb embryos transiently overexpressing wnt2bb, determined by hepatic Prox1 expression. (C) Live picture of injected embryo 2 hours after heat-shock. mCherry indicates cells in which the hsp70:mCherry-2A-wnt2bb construct was activated. (D) Whole-mount mRNA in situ hybridization for wnt2bb showing ectopic activation 3 hours after heat-shock. Staining was stopped before endogenous expression was detectable. (E-H2) Cell proliferation rates are increased in embryos in which wnt2bb (F,F2) or wnt2 (H,H2) have been ectopically activated; numbers indicate count of individual Prox1- and BrdU-positive cells. Liver anlage (broken line) is expanded posteriorly in embryos with excess wnt2bb (F,F2) and wnt2 (H,H2) compared with controls (E,E2); inter-renal primordium (arrowhead) serves as landmark. (G) Proliferation rates in controls, hsp70:mCherry-2A-wnt2bb and hsp70:mCherry-2A-wnt2 embryos determined by Prox1 and BrdU labelling. (I) Cell proliferation in the LPM is decreased in prt/wnt2bb embryos at 30 hpf and increased in embryos after its transient activation at 18 hpf; proliferating cells were determined by BrdU and Tg(XlEef1a1:GFP)s854 to exclude the endoderm. (J) cyclinD1 and myca expression 4 hours after transient wnt2bb and wnt2 overexpression at 26 hpf; controls represent siblings exposed to the same heat-shock regime to determine the effect specific to Wnt overexpression. (C) Lateral view, (D) dorsal view, (E-H2) ventral views of projections of confocal stacks; all anterior towards the top. (B,G,I,J) Standard errors and P values are shown (*Pd0.05; **P<0.001; ***P<2.10-8).

Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development