IMAGE

Fig. 6

ID
ZDB-IMAGE-101206-34
Genes
Source
Figures for Li et al., 2010
Image
Figure Caption

Fig. 6 Altered Id3 expression levels result in a decreased number of TH+ cells at the PT area. A: TH in situ hybridization shows that the number of TH+ cells is decreased in Id3 mRNA-injected larvae. B: Quantification of the data shown in A. The graph shows the percentage of phenotypic embryos with reduced number of TH+ cells. C: TH in situ hybridization analysis in the larvae injected with control morpholino, Id3 morpholino, or Id3 morpholino with ΔId3 mRNA (Id3 mRNA lacking paired sequence with morpholino). The number of TH+ cells is also decreased in embryos injected with Id3 morpholino. Co-injection with ΔId3 mRNA can rescue these defects. D: Quantification of the data shown in C. Also the number of embryos with defects was quantified. E: In situ hybridization of DAT in manipulated embryos of 30 hours postfertilization (hpf). The signals of DAT are decreased in either Id3 mRNA- or MO-injected embryos. F: Quantitative data shown in E. The number of embryos with defects was quantified. Inserts in A, C, E are magnified views of the stippled boxes in white representing part of the diencephalon. Arrows indicate the PT with reduced TH+ cells. *P < 0.05. **P < 0.01. TH, tyrosine hydroxylase; MO, morpholino; PT, posterior tuberculum; DAT, dopamine transporter.

Figure Data
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dev. Dyn.