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Fig. 3 Expression pattern of ihha, shha, ptc1, and ptc2 in swimbladder as detected by WISH. (A–C) Expression of shha. Starting from 48 hpf (A) and up to 72 hpf (B), expression of shha can be found in the epithelium of the swimbladder (sb) and gut (g). A cross section of swimbladder of a 48-hpf embryo (C) indicates shha expression in the swimbladder epithelium (e) and gut (g), in addition to that in floor plate (fp). The level of section in (C) is shown by a vertical line in (A). (D–F) Expression of ihha. Similar pattern of ihha expression at 48 hpf and 72 hpf was found along the gut, pneumatic duct and swimbladder, with stronger expression in the anterior pharynx. A cross section of swimbladder of a 72-hpf embryo (F) at the level shown in (E) indicates epithelial specific ihha expression. (G,H) Expression of ptc1 (G) and ptc2 (H) at 48 hpf. (I) Cross section of a 60-hpf embryo to show ptc2 expression in the mesenchyme of the swimbladder and gut. Abbreviations: e, epithelium; fp, floor plate; g, gut; m, mesenchyme; n, notochord; pd, pneumatic duct; ph, pharynx; sb, swimbladder. Scale bars: 250 mm (A–T) and 100 μm (U–X).
Reprinted from Developmental Biology, 331(2), Winata, C.L., Korzh, S., Kondrychyn, I., Zheng, W., Korzh, V., and Gong, Z., Development of zebrafish swimbladder: the requirement of Hedgehog signaling in specification and organization of the three tissue layers, 222-236, Copyright (2009) with permission from Elsevier. Full text @ Dev. Biol.