IMAGE

Fig. 3

ID
ZDB-IMAGE-090527-4
Genes
Antibodies
Source
Figures for Vitorino et al., 2009
Image
Figure Caption

Fig. 3 Vsx1 expression in the zebrafish retina. (A-D)vsx1 in situ hybridization in 24 hours post-fertilization (hpf) (A), 46 hpf (B), 62 hpf (C) and 80 hpf (D) Tg(vsx1:GFP) transgenic embryos shows complete co-localisation of vsx1 RNA and Vsx1 driven GFP expression. (E-I)Whole-mount lateral view of the Tg(vsx1:GFP) retina of zebrafish embryos counterstained with DAPI (blue) reveals expression of green fluorescent protein (GFP) in a subpopulation of retinal progenitor cells from 24 to 48 hpf as well as differentiated neurons at 72 and 96 hpf. (H-L)Cryosections through the central retina of Tg(vsx1:GFP) reveal GFP expression in cell bodies in the outer INL and processes in the OPL and IPL (arrows). GFP is also expressed in a few cells in the inner half of the INL (some indicated by asterisks). (J-L) Tg(vsx1:GFP) retinas at 80 hpf labelled with protein kinase C (PKC)β1 (J), calretinin (K) and Sox2 (L). Vsx1:GFP cells co-label with PKC-positive bipolar cells and calretinin-positive amacrine cells, but not with Sox2-labelled Müller cells. GCL, ganglion cell layer; INL, inner nuclear layer; ipl, inner plexiform layer; L, lens; ONL, outer nuclear layer; opl, outer plexiform layer. Scale bars: (A-I) 100 μm; (J-L) 40 μm.

Figure Data
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Neural Dev.