IMAGE

Fig. 9

ID
ZDB-IMAGE-090527-10
Genes
Source
Figures for Vitorino et al., 2009
Image
Figure Caption

Fig. 9 Ath5 progenitor cells arise from Vsx2 progenitors that lose Vsx2:GFP expression. (A, B) Images of live double transgenic Tg(vsx2:GFP/ath5:GapRFP) whole-mount retinas at different time points of retina development indicated as hours post-fertilisation (hpf). (A) At 28 hpf, a few cells in the retina no longer express green fluorescent protein (GFP) driven by vsx2. Some (white arrows), but not all of these Vsx2:GFP-negative cells express Ath5:RFP. (A′-B′) As development progresses, the number of cells that express Ath5:RFP increase as the number of Vsx2:GFP cells diminishes. Ath5-expressing, Vsx2:GFP-negative cells divide apically and many of the differentiating cells can be seen to settle in the developing ganglion cell layer, which becomes devoid of Vsx2:GFP cells. Images are from the movie in Additional data file 5. (C-F) Double label from a Tg(ath5:RFP;vsx2:GFP) retina in which an Ath5:RFP progenitor divides once to produce one daughter that differentiates as an RGC. (E′-F′) Vsx2 expression can be seen to be downregulated in this Ath5:RFP progenitor (whose soma is marked by the white dot). Scale bars: (A) 16 μm; (B) 27 μm; (C-F) 20 μm.

Figure Data
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Neural Dev.