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Fig. 3 Initiation of pdx-1 and insulin expression in the pancreatic primordium. (A–H) Dorsal and (I–N) lateral views of wild-type zebrafish embryos after in situ hybridization with pdx-1 (left panels) or insulin (right panels) antisense riboprobe at the (A, B) 10-somite stage; (C, D) 12-somite stage; (E, F) 14-somite stage; (G, H) 18-somite stage; (I, J) 20-somite stage; (K, L) 24-somite stage; and (M, N) 24 hpf. (O) 24 hpf embryo after in situ hybridization with both pdx-1 and insulin; n, notochord. The yolk was manually removed; anterior is to the left. Scale bar, 100 μm (N).
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Reprinted from Developmental Biology, 230(2), Biemar, F., Argenton, F., Schmidtke, R., Epperlein, S., Peers, B., and Driever, W., Pancreas development in zebrafish: early dispersed appearance of endocrine hormone expressing cells and their convergence to form the definitive islet, 189-203, Copyright (2001) with permission from Elsevier. Full text @ Dev. Biol.