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Fig. S3 Syn4 is required for NC migration. Migration of NC was examined in vivo using the transgenic zebrafish line sox10:egfp. (A,B) Embryo injected with control MO, showing normal migration of cephalic NC at 24 hpf. (C,D) Embryo injected with syn4 MO2 showing inhibition of NC migration at 24 hpf. The inhibition of NC migration is similar to that produced by syn4 MO1 (see Fig. 5F-J). (E) Dose-dependent effect of syn4 MO1 or MO2 on neural crest migration. (F) The mix of syn4 MO1 + syn4 MO2 has an additive effect on NC migration compared with injection of each single morpholino (n=79).
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