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Fig. S1 sox32 mRNA does not perturb normal endoderm development. A single cell was injected with sox32 mRNA and rhodamine dextran at the 32-cell stage as outlined in Materials and methods (A, C, E, G, I, K), or left uninjected (B, D, F, H, J, L). Embryos were fixed and assayed for expression of insulin at 24 hpf (A, B), p48 at 30 hpf (C, D), sid4 at 48 hpf (E, F), pdx1 at 48 hpf (G, H), insulin at 72 hpf (I, J) and carbA at 72 hpf (K, L) by whole mount in situ hybridization. All embryos are in lateral view with anterior to the left.
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Reprinted from Developmental Biology, 304(1), diIorio, P., Alexa, K., Choe, S.K., Etheridge, L., and Sagerström, C.G., TALE-Family homeodomain proteins regulate endodermal sonic hedgehog expression and pattern the anterior endoderm, 221-231, Copyright (2007) with permission from Elsevier. Full text @ Dev. Biol.