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Fig. 4 Exogenous RA Treatment Proximalizes the Pronephros. (A) Expression of segment markers and mhc at 24 hpf in wild-type embryos incubated with either DMSO (control) or RA at the indicated dosage and times during development, by double whole-mount in situ hybridization. Brackets and arrows indicate expression domains. Abbreviations are: %e (percent epiboly), s (somite stage). Dorsal views are shown, with anterior to the left, with the exception of lateral views for gata3 expression. (B) Summary of nephron segmentation with respect to embryo somite number for each RA experiment.
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