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Fig. 3 Temporal expression pattern of wt1a and wt1b during early zebrafish development. Analysis was performed by reverse transcriptase-polymerase chain reaction (PCR) using embryos of the stages indicated. Sizes of the amplified products were 520 bp for wt1a, 560 bp for wt1b, and 400 bp for β-actin, which was used as a reference. To verify the absence of genomic DNA contamination, an aliquot of the 24 hours postfertilization RNA sample was used for PCR without reverse transcription (-rt). h, hours postfertilization.
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