|
Fig. 3 Tenascin-C mRNA is expressed in adaxial cells and other cell types. Cross-sections of trunk regions of zebrafish embryos are shown. Dorsal is up. A-C: At 16 hours postfertilization (hpf), tenascin-C mRNA distribution (A) and immunolabeling for adaxial cell marker F59 (B) overlap (overlay in C) in dorsoventral rows of cells (arrows in A,B) adjacent to the notochord (n) and ventral spinal cord (sc). Hypochord cells (arrowhead in A) also express tenascin-C mRNA. D-F: At 24 hpf, tenascin-C mRNA (arrows in D) is found in weakly F59-immunopositive (E) myotomal cells, putative neural crest cells (asterisks in D), and hypochord (arrowhead in D). Putative migrating sclerotomal cells (small arrows in D) and laterally migrated adaxial cells (arrows in E) do not express the message (see overlay in F), with the possible exception of the muscle pioneer cells (asterisks in E). Scale bar = 25 μm in F (applies to A-F).