Tenascin-C is involved in motor axon outgrowth in the trunk of developing zebrafish
- Schweitzer, J., Becker, T., Lefebvre, J., Granato, M., Schachner, M., and Becker, C.G.
- Developmental Dynamics : an official publication of the American Association of Anatomists 234(3): 550-566 (Journal)
- Registered Authors
- Becker, Catherina G., Becker, Thomas, Granato, Michael, Lefebvre, Julie, Schachner, Melitta, Schweitzer, Jörn
- extracellular matrix, chondroitin sulfate proteoglycans, horizontal myoseptum, primary motor neurons, adaxial cells, stumpy, topped, Danio rerio
- MeSH Terms
- Amino Acid Sequence
- Cell Differentiation
- Cloning, Molecular
- DNA, Complementary/genetics
- Epidermal Growth Factor/genetics
- Epidermal Growth Factor/metabolism
- Gene Expression Regulation, Developmental
- Molecular Sequence Data
- Motor Neurons/chemistry
- Motor Neurons/cytology
- Motor Neurons/metabolism*
- RNA, Messenger/genetics
- Sequence Alignment
- Sequence Homology, Amino Acid
- 16110513 Full text @ Dev. Dyn.
Schweitzer, J., Becker, T., Lefebvre, J., Granato, M., Schachner, M., and Becker, C.G. (2005) Tenascin-C is involved in motor axon outgrowth in the trunk of developing zebrafish. Developmental Dynamics : an official publication of the American Association of Anatomists. 234(3):550-566.
Motor axons in the trunk of the developing zebrafish exit from the ventral spinal cord in one ventral root per hemisegment and grow on a common path toward the region of the horizontal myoseptum, where they select their specific pathways. Tenascin-C, a component of the extracellular matrix, is concentrated in this choice region. Adaxial cells and other myotomal cells express tenascin-C mRNA, suggesting that these cells are the source of tenascin-C protein. Overexpressing an axon repellent fragment containing the cysteine-rich region and the epidermal growth factor-like repeats of tenascin-C led to retarded growth of ventral motor nerves between their spinal exit point and the horizontal myoseptum. Injection of a protein fragment containing the same part of tenascin-C also induced slower growth of motor nerves. Conversely, knock down of tenascin-C protein resulted in abnormal lateral branching of ventral motor nerves. In the zebrafish unplugged mutant, in which axons display pathfinding defects in the region of the horizontal myoseptum, tenascin-C immunoreactivity was not detectable in this region, indicating an abnormal extracellular matrix in unplugged. We conclude that tenascin-C is part of a specialized extracellular matrix in the region of the horizontal myoseptum that influences the growth of motor axons.
Genes / Markers
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Engineered Foreign Genes