Gene Trap Construct

Gt(GBT-R15)

ID

ZDB-GTCONSTRCT-100121-2

Name

Gt(GBT-R15)

Previous Names

pGBT-R15
R15 (1)
gene-breaking transposon R15 (1)
Gt3(LOXP-mRFP1-LOXP)

Type

engineered_plasmid

Regulatory Regions

None

Coding Sequences

mRFP1 (1)

[Em λ][Ex λ]

Fpbase:mRFP1

Contains Sequences

LOXP (1)
T2M (1)

Note

Construct contains a monomeric RFP reporter (specifically *mRFP1, which is mRFP1 with the start codon removed).

Plasmid Map

Genomic Features

That Utilize Gt(GBT-R15) Construct

Genomic Feature	Affected Genomic Regions
is22Gt	appa
is23Gt	aplp2
mn0001Gt	casz1
mn0002Gt	sorbs2b
mn0005Gt	itgb1b
mn0006Gt
mn0008Gt
mn0010Gt	cdh11
mn0012Gt
mn0018Gt
mn0029Gt
mn0043Gt	cd99l2
mn0083Gt
mn0098Gt
mn31Gt	gabbr1b

Transgenics

That Utilize Gt(GBT-R15) Construct

Fish	Affected Genomic Regions	Phenotype	Gene Expression
ak2^hg14/hg14; sorbs2b^mn0002Gt; s356tTg	ak2 sorbs2b		Fig. 4. from Rissone et al., 2019
aplp2^is23Gt/+; is1Tg/+	aplp2		Fig. 5 from Liao et al., 2012
aplp2^is23Gt/+; tnnt2a^mn0031Gt/+; is1Tg/+	aplp2 tnnt2a	Fig. S7 from Liao et al., 2012	Fig. S7 from Liao et al., 2012
aplp2^is23Gt/+; tnnt2a^{mn0031Gt/mn0031Gt}; is1Tg/+	aplp2 tnnt2a	Fig. S7 from Liao et al., 2012	Fig. S7 from Liao et al., 2012
aplp2^{is23Gt/is23Gt} (WIK)	aplp2
appa^is22Gt/+; is1Tg/+	appa		Fig. 4 from Liao et al., 2012
appa^is22Gt/+; tnnt2a^mn0031Gt/+; is1Tg/+	appa tnnt2a	Fig. S7 from Liao et al., 2012	Fig. S7 from Liao et al., 2012
appa^is22Gt/+; tnnt2a^{mn0031Gt/mn0031Gt}; is1Tg/+	appa tnnt2a	Fig. S7 from Liao et al., 2012	Fig. S7 from Liao et al., 2012
appa^{is22Gt/is22Gt} (WIK)	appa
appa^{ua5006/ua5006}; appa^{is22Gt/is22Gt}	appa	text only from Kanyo et al., 2020
appa^{ua5007/ua5007}; appa^{is22Gt/is22Gt}	appa	text only from Kanyo et al., 2020
appa^{ua5008/ua5008}; appa^{is22Gt/is22Gt}	appa	text only from Kanyo et al., 2020
bag3^xu083/xu083; sorbs2b^{mn0002Gt/mn0002Gt}	bag3 sorbs2b	Fig. 1 from Ding et al., 2022
casz1^mn0001Gt/+ (AB)	casz1
casz1^{mn0001Gt/mn0001Gt}	casz1		Fig. 2 from Clark et al., 2011
cd99l2^mn0043Gt/+ (AB)	cd99l2
cd99l2^{mn0043Gt/mn0043Gt}	cd99l2		Fig. 4 from Clark et al., 2011
cdh11^{mn0010Gt/mn0010Gt}	cdh11
gabbr1b^mn31Gt/+	gabbr1b	2 figures from Petzold et al., 2009	Fig. 4 from Petzold et al., 2009
gabbr1b^mn31Gt/+ (AB)	gabbr1b
gabbr1b^{mn31Gt/mn31Gt}	gabbr1b	2 figures from 2 publications	Fig. 4 from Petzold et al., 2009
itgb1b^mn0005Gt/+ (AB)	itgb1b
itgb1b^{mn0005Gt/mn0005Gt}	itgb1b		Fig. 4 from Clark et al., 2011
mn0006Gt/+ (AB)
mn0008Gt/+ (AB)
mn0012Gt/+ (AB)
mn0018Gt/+ (AB)
mn0029Gt/+ (AB)
mn0083Gt/+ (AB)
mn0098Gt/+ (AB)
sorbs2b^mn0002Gt/+	sorbs2b	2 figures from Ding et al., 2016
sorbs2b^mn0002Gt/+ (AB)	sorbs2b
sorbs2b^{mn0002Gt/mn0002Gt}	sorbs2b		Fig. 2 from Clark et al., 2011
sorbs2b^{mn0002Gt/mn0002Gt}; pd10Tg	sorbs2b	Fig. S3 from Ding et al., 2020	Fig. S3 from Ding et al., 2020
sorbs2b^mn0002Gt; s356tTg	sorbs2b		Fig. 4. from Rissone et al., 2019

Sequence Information

Other Construct Pages

zfishbook:R15 (1)

Citations