Gene Trap Construct
Tg(GAL4-VP16,UAS:EGFP)
- ID
- ZDB-GTCONSTRCT-070314-1
- Name
- Tg(GAL4-VP16,UAS:EGFP)
- Previous Names
-
- SAGVG (1)
- Gt(Gal4-VP16,UAS:EGFP)
- Gt(Gal4-VP16;UAS:EGFP)
- Tg(Gal4-VP16,UAS:EGFP)
- Type
- engineered_plasmid
- Regulatory Regions
- Coding Sequences
- [Em λ][Ex λ]
- Fpbase:EGFP
- Contains Sequences
- None
- Note
-
A Tol2 transposon-based, bipartite construct consisting of a Gal4-VP16 gene/enhancer trap as well as a cis-linked UAS-regulated eGFP reporter. The vector contains a splice acceptor sequence derived from the rabbit beta-globin gene (275 base pairs of intron 2 and 45 base pairs of exon 3) which was amplified from the pT2KXIG”IN vector (Urasaki et al., 2006) and cloned upstream of tandem Gal4-VP16 and UAS:eGFP elements (Koster and Fraser, 2001). The entire unit was subcloned into the pT2KXIG”IN vector to be flanked by a 535 bp right Tol2 arm and a 517 bp left Tol2 arm.
Plasmid Map
None
Genomic Features
That Utilize Tg(GAL4-VP16,UAS:EGFP) Construct
Transgenics
That Utilize Tg(GAL4-VP16,UAS:EGFP) Construct
| Fish | Affected Genomic Regions | Phenotype | Gene Expression |
|---|---|---|---|
| as38Tg |
|
Fig. 3
from
Liu et al., 2020
|
|
| c215Tg (AB) |
|
||
| c218Tg (AB) |
|
Fig. 1 from Goll et al., 2009 | |
| c223Tg (AB) |
|
Fig. 1 from Goll et al., 2009 | |
| c223Tg/+; c264Tg/+ |
|
Fig. 5 from Davison et al., 2007 |
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Sequence Information
Other Construct Pages
None
Citations