Figure 6.

COMMD9 regulates drug stimulation-mediated hypercoagulability. (A) Western blot analysis of P-selectin and COMMD9 expression in HUVECs and HHSECs following COMMD9 knockdown or overexpression, followed by 1 U/mL thrombin incubation. (B) Schematic representation of the platelet–endothelial adhesion assay and quantification of adhesion rates. (C) HHSECs with COMMD9 knockdown or overexpression were incubated with platelets from healthy donors under basal conditions. Adhesion was assessed by flow cytometry (N = 3 biological replicates). (D) O-dianisidine staining of larvae showing an increased or decreased blood cell accumulation in the caudal veins (black dashed line) after commd9 inhibition or overexpression, respectively, followed by 1.5 µM PHZ incubation for 24 h. Scale bar, 100 μm. N = 12–16 larvae per group. All micrographs show lateral views, with anterior to the left and dorsal above. The results are shown as mean ± SD, ****P < 0.0001; ***P < 0.001; **P < 0.01; *P < 0.05; n.s., not significant.