FIGURE

Fig. 5

ID

ZDB-FIG-260716-58

Publication

Wang et al., 2026 - Checkpoint kinase 2 coordinates autophagy activation and Aurora kinase A degradation to regulate primary cilia for cell invasion

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Fig. 5

CHEK2 promotes PDAC cell invasion upon Gln deprivation. A-E Gln deprivation activates CHEK2 and promotes primary ciliation, migration, and invasion. A CHEK2 was phosphorylated in PANC-1 cells under Gln deprivation. Extracts of PANC-1 cells under full-medium (FM) or Gln deprivation (-Q) were analyzed by Western blotting with antibodies against phosphorylated CHEK2 (p-CHEK2), CHEK2, and actin. B Quantitative results for the relative intensity of p-CHEK2/CHEK2 in A. Quantitative results of (C) ciliated, (D) migrated, or (E) invaded cells in full-medium (FM) or Gln-deprived medium (-Q). D-H CHEK2 promotes PDAC invasion under glutamine deprivation. F-J Depletion of CHEK2 reduced primary cilia, migration, and invasion under Gln deprivation. F Extracts of PANC-1 cells in the absence or presence of siCHEK2 were analyzed by Western blotting with antibodies against CHEK2 and Ku70. G Quantitative results for the relative intensity of CHEK2 in F. H-J Quantitative results of (H) ciliate, (I) migrated, or (J) invaded cells in control or CHEK2-deficient (siCHEK2) cells under Gln deprivation (-Q). **p < 0.01, ***p < 0.001. These results are mean ± SD from three independent experiments

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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