FIGURE

Figure 5

ID

ZDB-FIG-260316-83

Publication

Wang et al., 2026 - Zebrafish PRL-3 Regulates Yolk Syncytial Layer Integrity and Actomyosin Contractility During Epiboly

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Figure 5

Whole-mount F-actin and nuclear staining reveals abnormal YSL distribution and premature actomyosin ring formation in zfPRL-3 morphants. Representative images of embryos injected with 4ng control morpholino (MO) or zfPRL-3 MO and stained for F-actin (phalloidin) and nuclei (DAPI; 4′,6-diamidino-2-phenylindole). Panels are grouped by staining modality to highlight different readouts: nuclei (DAPI) together with F-actin to provide positional context for YSL nuclei, versus F-actin–only staining to better resolve the actomyosin ring. Brackets in panels (A,B,E,F) indicate the region corresponding to the yolk syncytial layer (YSL). Arrowheads in panels (D,G,H) indicate the actomyosin ring. (A–D) Control morphants. (E–H) zfPRL-3 morphants. In zfPRL-3 morphants at ~60% epiboly, bracket in panel (F) highlight dispersed external YSL-associated nuclei, and arrowheads in panel (G) indicate the early appearance of the actomyosin ring relative to controls. Scale bars = 250 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO1-ptp4a3a
Knockdown Reagent:	MO1-ptp4a3a
Observed In:	external yolk syncytial layer nucleus margin actomyosin contractile ring
Stage Range:	1k-cell to 75%-epiboly

Phenotype Detail

Acknowledgments

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