FIGURE

Figure 3

ID

ZDB-FIG-260313-14

Publication

Tarahomi et al., 2025 - Transcriptomic identification and developmental mapping of nrg3b expression in zebrafish

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Figure 3

Validation of nrg3b expression at 5 dpf. A, RT-qPCR validation of nrg3b expression at 1, 3, and 5 dpf. Expression was normalized to day 1 using rpl13a as the reference gene. Data represent the mean fold-change ± SEM (n = 3 biological replicates × 3 technical replicates per time point). One-way ANOVA showed a significant effect of the developmental stage (F_(2,24)=9.99, p < 0.001). Post hoc Dunnett’s test indicated significant upregulation on days 3 and 5 vs. day 1. B, Detection of nrg3b expression in embryos. WISH in 5 dpf zebrafish embryos using nrg3b, lbx1a (positive control (PC)), and no-probe (negative control (NC)). Lateral views (left) show whole-body expression; dorsal views (right) show the head region. Scale bars: 500 μm for the whole body, 100 μm for the dorsal views.

Expression Data

Genes:	lbx1a nrg3b
Fish:	WT
Anatomical Terms:	hindbrain whole organism
Stage Range:	Prim-5 to Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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