FIGURE

FIGURE 4

ID

ZDB-FIG-260129-38

Publication

Han et al., 2026 - Multiple susceptibility enhancer variants increasing ADD3 expression predisposes to biliary atresia risk

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FIGURE 4

Knockdown or overexpression of add3a induces biliary dysfunction in zebrafish. (A) Lateral view of 5 dpf zebrafish larvae after ingestion of PED-6. The gallbladders of larvae injected with add3a mRNA or MOs were smaller compared to control embryos. (B) Quantitative analysis showed that the accumulation of PED-6 in the gallbladders of mRNA-injected larvae and morphants were significantly reduced compared to control larvae. Scale bar = 100 μm. Chi-square test was used. *P < 0.05; **P < 0.01; ***P < 0.001. (C) Confocal projection of 5 dpf larvae stained for cytokeratin 18, comparing control larvae, mRNA-injected larvae, and add3a morphants. The intrahepatic bile ducts in both mRNA-injected larvae and add3a morphants appeared sparser than in controls. n = 10 for each group. (D) Confocal projection of 5 dpf larvae stained for cytokeratin 18, showing that the gallbladders of both mRNA-injected larvae and add3a morphants were smaller than those of controls. n = 10 for each group. Gallbladder cells are indicated by white dots.

Expression Data

Antibody:	Ab8-krt18
Fish:	AB AB + MO2-add3a AB + MO4-add3a
Knockdown Reagents:	MO2-add3a MO4-add3a
Anatomical Terms:	gall bladder intrahepatic bile duct epithelial cell
Stage:	Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO2-add3a AB + MO4-add3a
Knockdown Reagents:	MO2-add3a MO4-add3a
Observed In:	intrahepatic bile duct liver A2-type glycerophospholipase activity
Stage:	Day 5

Phenotype Detail

Acknowledgments

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