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dnmt3ba deficiency reduces methylation and expression level of Integrin signaling genes. A The genomic distribution of hypermethylated and hypomethylated DMRs in dnmt3ba morphants compared to controls. B Venn diagram exhibiting the overlap between downregulated genes and genes with Hypo-DMRs in gene body regions in dnmt3ba morphants. C KEGG analysis for the overlapping genes in (B). D qRT-PCR analysis of itgα3b, itgα7, and lamb2 expression in HECs in control and dnmt3ba morphants at 36 hpf. Three independent experiments were conducted. E qRT-PCR analysis of gene expression in HECs in WT and dnmt3ba mutants at 36 hpf. Three independent experiments were conducted. F–I Bisulfite sequencing analysis and quantification of DNA methylation levels at DMRs in itgα3b and itgα7 in HECs of control and dnmt3ba morphants at 36 hpf. Three independent experiments were conducted. J–M Bisulfite sequencing analysis and quantification of DNA methylation levels at DMRs in itgα3b and itgα7 in ECs/HECs of WT or dnmt3ba mutants at 36 hpf. Three independent experiments were conducted. Statistical significance was determined by a Two-tailed Student’s t-test in (D, E) and a Two-tailed Fisher’s exact test in (G, I, K, M). Data are presented as mean ± SD.
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