Fig. 6

Long-term depletion of ROS reduces tissue stiffness. (A) Schematics of tissue stiffness measurement of MO-injected larvae at 3 dpf. (B) Young’s modulus measurements of the zebrafish tailfin of the duox morphant (n = 20) and p53 control (n = 33) groups. The red asterisk indicates the midrange in the p53 group. Data are median ± IQR from three independent experiments (N = 3). Kolmogorov-Smirnov test. (C) Schematics of an in silico linear elasticity simulation of the zebrafish tailfin postamputation. The third principal stress at the wound margin was simulated with a node-wise prescribed displacement derived from experimental data. (D) The displacement of the tailfin edge tip changes over time in both experimental data and in silico-fitted data of MO-injected groups (SSE: duox + p53: 0.01, p53: 0.6724). (E) Simulated principal stress at the zebrafish tailfin wound margin over time for the MO-injected groups. (F) Principal stress at the zebrafish tailfin wound margin at 900 and 3200 s postamputation and the corresponding displacement of the MO-injected groups. (G) The displacement of the tailfin edge over time in both experimental data and in silico-fitted data of the DPI-treated and DMSO control groups (SSE: DMSO, 2.75; DPI, 1.0576). (H) Simulated principal stress at the zebrafish tailfin wound margin over time for the DPI-treated and DMSO control groups. (I) Principal stress at the zebrafish tailfin wound margin at 900 and 3200 s postamputation, along with the corresponding displacement, of the DPI-treated and DMSO control group.