Fig. 2

H2O2 restores wound relaxation in ROS-depleted wounds. (A) Workflow of H2O2 rescue assay of the DPI-treated embryo and DMSO control. Embryos were supplemented with 3 mM H2O2 immediately after amputation, while the DPI was maintained in E3. (B) Representative images of wounds in the DPI-treated larvae with or without H2O2 supplement at 0, 10, 20, and 30 mpa. Scale bar, 50 μm. (C) Wound widths at the indicated time points were normalized to 0 mpa for the DPI-treated (n = 9) and H2O2 rescue (n = 9) groups. Data are mean ± SE, pooled from three independent experiments (N = 3). (D) Workflow of H2O2 rescue assay with duox knockdown. One-cell stage embryos were injected with either duox + p53 morpholino. Embryos at 3 dpf were wounded and supplemented with 3 mM H2O2 immediately postamputation. (E) Representative images of wounds in duox + p53-injected groups with or without H2O2 supplement at 0, 5, 10, and 15 mpa. Scale bar, 50 μm. (F) Wound widths at the indicated time points were normalized to 0 mpa for duox + p53-injected (n = 8) and H2O2 rescue (n = 9) groups. Data are presented as median ± IQR, pooled from three independent experiments (N = 3). (C) Unpaired t-test. (F) Mann-Whitney test.