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AIBP regulates LEC caveolae. a TEM analysis of control and recombinant AIBP-treated hLECs. Arrows depict caveolae. b Quantification of caveolae in (a). n = 35 (control) and n = 36 (AIBP) cells. Data are Mean ± SE; unpaired two-sided t-test with Welch’s correction. c TEM analysis of caveolae in ECs of the cardinal vein from control and apoa1bp2−/− zebrafish. Arrows indicate closed caveolae and arrowheads show open caveolae. d Quantification of caveolae in cardinal vein ECs. n = 27 (control) and n = 29 (apo1bp2−/−) cells. Data are Mean ± SE; unpaired two-sided t-test with Welch’s correction. e Scheme illustration of 4 hydroxy-tamoxifen (4OHT)-induced ubiquitous mEos2-APOA1 expression. f Representative images of control and mEos2-APOA1 expressed embryos after 4OHT treatment. Embryos were imaged at 2 dpf. The white dashed line demarcates the control animals. The results are representative of 3 independent repeats. g Photoconversion and vascular circulation of mEos2-APOA1. The head regions of embryos were exposed to UV light for 1 min to induce photoconversion. h Analysis of mEos2-APOA1 secretion. The specified tail region was imaged, and the maximum RFP-to-GFP signal ratio within the ISV lumen was quantified. n = 8 intersegmental vessels from 2 embryos. Data are presented as mean ± SEM, analyzed using one-way ANOVA with Tukey’s post-hoc test. i Maxi-projection confocal images of Prox1+ cells in the apoa1bp−/−; fli1a:egfp zebrafish with control (mEos2-APOA1) and mEos2-APOA1 overexpression (ubi:Gal4-ERT2+mEos2-APOA1) at 4 dpf and immunostained with GFP (green) and Prox1 (red) antibodies. Arrowheads show the Prox1+ LECs in TD. j Quantitative data of Prox1+ LEC in TD (7 somites). n = 10 (mEos-APOA1) and n = 11 (ubi:Ert2-Gal4; mEos-APOA1) embryos. Data are Mean ± SE; unpaired two-sided t-test with Welch’s correction. CV cardinal vein. Scale bar: 400 nm in a and 500 nm in c; 100 µm in (f, g, i). Source data are provided as a Source Data file.
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