Figure 6

cGAS-STING pathway rescues premature aging phenotypes.

(A) Quantification of male fertile capacity (n_WT = 15, n_tert-/- = 10, n_sting-/- = 14, n_{tert-/- sting-/-} = 13), WT vs tert-/- p = 0.000001, sting-/- vs tert-/- p = 0.000001, WT vs tert-/- sting-/- p = 0.011, sting-/- vs tert-/- sting-/- p = 0.0001, tert-/- vs tert-/- sting-/- p = 0.002). (B) Representative images of adult zebrafish and quantification of aging phenotypes scored as kyphosis and cachexia (n_WT = 10, n_tert-/- = 12, n_sting-/- = 11, n_{tert-/- sting-/-} = 12, WT vs tert-/- p = 0.000001, sting-/- vs tert-/- p = 0.000001, WT vs tert-/- sting-/- p = 0.001, sting-/- vs tert-/- sting-/- p = 0.003, tert-/- vs tert-/- sting-/- p = 0.000001). (C) Quantification of seminoma (n_WT = 17, n_tert-/- = 16, n_sting-/- = 21, n_{tert-/- sting-/-} = 18, WT vs tert-/- p = 0.012, sting-/- vs tert-/- p = 0.002, tert-/- vs tert-/- sting-/- p = 0.027). (D) Quantification of weight in adult zebrafish (n_WT = 12, n_tert-/- = 13, n_sting-/- = 11, n_{tert-/- sting-/-} = 13, WT vs tert-/- p = 0.0002, sting-/- vs tert-/- p = 0.004, tert-/- vs tert-/- sting-/- p = 0.012). (E) Quantification of survival n_WT = 24, n_tert-/- = 38, n_sting-/- = 32, n_{tert-/- sting-/-} = 37, WT vs tert-/- p = 0.008, sting-/- vs tert-/- p = 0.005, tert-/- vs tert-/- sting-/- p = 0.010). Data were presented as the mean ± s.e.m. *p < 0.05; **p < 0.01, ***p < 0.001, using a one-way ANOVA and post hoc Tukey test. Seminoma occurrence and survival data were analyzed using log-rank tests, **p < 0.01, ***p < 0.001.