Fig. 4

Effect of Socs3a ablation on posterior lateral line neuromast formation. (A,B,D,E,H,I,K,L,N,O,Q,R) Representative images of Socs3a wildtype (WT) and knockout (KO) embryos analyzed using WISH with sdf1a (A,B) and cxcr4b (D,E) at 30 hpf, and eya1 (H,I) and atoh1a (K,L) at 3 dpf or stained with Mitotracker red, allowing visualization of posterior lateral line (PLL) neuromasts L1-8 at 3 dpf (N,O) and 4 dpf (Q,R), with scale bars of 100 µm shown. (C,F,G,J,M,P,S) Quantification of the area of sdf1a staining in the head, trunk and tail as described [31] (C), distance traveled by cxcr4b+ primordia (F), area of cxcr4b+ primordia (G), and number of eya1+ (J), atoh1a+ (M) or Mitotracker red stained (P,S) neuromasts, showing individual points along with the mean ± SEM and statistical significance (*** p < 0.001, ** p < 0.01, * p < 0.05; n = 10–15).