FIGURE

Fig. 2

ID

ZDB-FIG-250521-7

Publication

Xu et al., 2025 - Microglia-Derived IL-6 Promotes Müller Glia Reprogramming and Proliferation in Zebrafish Retina Regeneration

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Fig. 2

Intravitreous injection of zebrafish IL-6 activates Jak-Stat3 signaling and enhances the inflammatory response in injured zebrafish retinas. (A, B) The qPCR analysis showing the mRNA expression of Jak-Stat3 signaling component genes (stat3, il6st, il6r, socs3a, and socs3b) and inflammatory cytokines (il1b, il6, il11a, il11b, tnfa, and tnfb), in the uninjured control, or stab-injured retinas that received daily intravitreal IL-6 or PBS injection at 1 or 2 dpi. (C, D, E) IL-6 injection significantly increased the number and spatial distribution of IB4+ microglia at the injury site at 2 dpi. White *, site of the stab injury. (F) EdU immunofluorescence showing the cell proliferation in PBS control or IL-6-treated retinas at 4 days post injection. (G) Quantification of the total number of INL EdU+ cells in the retina at 4 days post injection. *, P < 0.05; **, P < 0.01, ***, P < 0.001 compared with PBS control. GCL, ganglion cell layer; INL, inner nuclear layer; ONL, outer nuclear layer.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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