FIGURE

Figure 4

ID
ZDB-FIG-250318-41
Publication
Garrigue et al., 2025 - Human Oncostatin M deficiency underlies an inherited severe bone marrow failure syndrome
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Figure 4

Osm-morphants show erythropoiesis defect.

(A) globin:GFP embryos were injected with control- or osm- morpholino (MO), and observed by fluorescence microscopy to measure their content in erythrocytes. Dashed rectangles highlight the part of zebrafish in which the cells are detected. Numbers in bottom left of images indicate the number of embryos displaying the indicated phenotype out of the total number of embryos analyzed. Scale bars: 100 μm. (B) The strong reduction of circulating erythrocytes (detected by gata1:DsRed marker) in osm-MO is rescued by overexpressing osm (noted + osm mRNA). Numbers in bottom left of images indicate the number of embryos displaying the indicated phenotype out of the total number of embryos analyzed. Scale bars: 200 μm. (C) 23 hpf globin:GFP embryos were stained for phospho-histone 3 (pH3), a marker of proliferation. Arrows indicate colocalization of pH3 and globin:GFP. Scale bar: 100 μm. (D) Fewer globin:GFP/pH3 double-positive cells are detected in osm-morphants, demonstrating a decrease in proliferating erythroid progenitors. Statistics derived from unpaired t tests with α = 0.05. **P < 0.01. (E) Quantitative PCR performed on sorted erythrocytes (GFP+ cells) at different stages show that they express high levels of osmr and osm transcripts. (F) Left: mpx:GFP embryos were injected with control- or osm- morpholino (MO) and observed by fluorescence microscopy to measure their content in neutrophils. Right: Fewer mpx:GFP cells are detected in osm-morphants, demonstrating a decrease in neutrophils. Statistics derived from unpaired t tests with α = 0.05. **P < 0.01.

Expression Data
Genes:
Fish:
Anatomical Term:
Stage Range: Prim-5 to Protruding-mouth

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Knockdown Reagent:
Observed In:
Stage Range: 26+ somites to Long-pec

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Journal of Clin. Invest.