FIGURE

FIGURE 2

ID

ZDB-FIG-250207-34

Publication

Toms et al., 2024 - A Novel De Novo Missense Variant in Netrin-1 (NTN1) Associated With Chorioretinal Coloboma, Sensorineural Hearing Loss and Polydactyly

Other Figures

All Figure Page

Back to All Figure Page

FIGURE 2

Ocular coloboma and sensory hair cell defects in ntn1a morphant zebrafish. (A) Fusion of the optic fissure was not complete in ntn1a morphant embryos at 56 h post‐fertilisation. The arrow indicates the ocular coloboma. (B) The lateral line neuromasts were visualised in control and ntn1a larvae at 5 days post‐fertilisation (dpf). Anti‐acetylated tubulin (AT, green) detected the hair cell bodies and kinocilia and Alexa Fluor 647 Phalloidin (red) stained the stereociliary hair bundles. The ntn1a neuromasts lacked hair cell bodies, hair bundles and kinocilia. (C) The stereociliary hair bundles of the anterior macula in the inner ear were visualised used Alexa Fluor 647 Phalloidin (white). The hair bundle morphology appeared to be normal in ntn1a at 5 dpf, but the hair bundles were reduced in number. (D) The bar charts show that the numbers of hair bundles per neuromast (n = 9, *p < 0.05) and per anterior macula (n = 5, ***p < 0.001) were significantly reduced in the ntn1a morphant compared with control larvae at 5 dpf. Scale bars = 10 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO5-ntn1a
Knockdown Reagent:	MO5-ntn1a
Observed In:	hair cell anterior macula stereocilium bundle hair cell posterior macula stereocilium bundle macula stereocilium bundle neuromast hair cell cell body neuromast hair cell kinocilium neuromast hair cell stereocilium bundle optic fissure
Stage Range:	Long-pec to Day 5

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Clin. Genet.