FIGURE

Fig. 5

ID

ZDB-FIG-241204-5

Publication

Burrows et al., 2024 - RAN Translation of C9orf72-Related Dipeptide Repeat Proteins in Zebrafish Recapitulates Hallmarks of Amyotrophic Lateral Sclerosis and Identifies Hypothermia as a Therapeutic Strategy

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Fig. 5

Hypothermic cooling reduces DPR expression in C9orf72-ALS/FTD cell models. (A) HEK293T, N2A and HeLa cells were transfected with control (C), sense (S), and antisense (AS) constructs and protein lysates were collected following 48 hours of hypothermic cooling (32°C). Western blot analysis and quantification of (B–D) V5-DPRs and (E–G) cold shock proteins CIRBP and RBM3. Data presented as mean ± SD. One-way ANOVA; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. ALS = amyotrophic lateral sclerosis; ANOVA = analysis of variance; CIRBP = cold-inducible RNA-binding protein; FTD = frontotemporal dementia; DPR = dipeptide repeat; SD = standard deviation. [Color figure can be viewed at www.annalsofneurology.org]

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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