FIGURE

Fig. S1

ID
ZDB-FIG-241009-8
Publication
Ugur et al., 2024 - VPS13B is localized at the interface between Golgi cisternae and is a functional partner of FAM177A1
Other Figures
All Figure Page
Back to All Figure Page
Fig. S1

Structure and localization of VPS13B. (A) Domain structure of human VPS13B. (B) Alphafold2 predicted structure of full-length human VPS13B; orange indicates the rod consisting of 13 RBG domains. (C) Alphafold2 prediction of the Jelly Roll (JR) domain of VPS13B alone on the left and overlayed with the jelly-roll fold of anaphase-promoting complex subunit Doc1p/Apc10 on the right. (D) Alphafold2 prediction of VPS13B internally tagged with Halo tag (magenta). (E) Snapshots of Golgi complex fragments of HeLa cells expressing VPS13B^Halo, ManII-RFP (a medial-Golgi marker), and either GalT-GFP (top panel) or Bet1-GFP (bottom panel) after a 10-min hypotonic shock. Scale bar = 1 µm. (F) Snapshots of Golgi complex and ER fragments of HeLa cells expressing VPS13B^Halo, VapB-RFP, and GalT-GFP after a 10-min hypotonic shock. Scale bar = 1 µm. (G) Top panel: HeLa cells co-expressing VapB-RFP and the N-terminal fragment of VPS13B (a.a. 1–586). Bottom panel: HeLa cells co-expressing VapB-RFP and the mutant N-terminal fragment (a.a. 1–586): VPS13BS559E, S560E, S563E. Scale bar = 10 µm. (H) COS7 cells expressing VPS13B^Halo and kept at 37°C (top panel) or shifted to 20°C for 30 min before fixation (bottom panel) and then stained with Lipid Tox. Scale bar = 5 µm.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Cell Biol.