FIGURE

Fig. 6

ID

ZDB-FIG-240709-16

Publication

Ghaffar et al., 2024 - Variants of NAV3, a neuronal morphogenesis protein, cause intellectual disability, developmental delay, and microcephaly

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Fig. 6

NAV3-WT mRNA injections rescued the phenotype in morphants as compared to variants harboring mRNAs. a Bar graph representation of morphant morphological phenotypes after injection of four different concentrations (250 pg, 500 pg, 750 pg, and 1000 pg) along with nav3 ATG_MO. The 500 pg concentration of NAV3-WT mRNA injection revealed the most significance rescue of normal class larvae. NS not-significant; ***p < 0.000250; and ****p < 0.00001. b Rescue experiments performed via co-injecting human NAV3WT or variants harboring mRNAs (500 pg) along with ATG_MO (8 ng) revealed significant rescue of normal developmental class in WT mRNA injected morphants compared to ATG_MO alone. The normal phenotype class in morphants from NAV3WT and the variants mRNAs co-injected were compared for statistical analysis (***p < 0.0001; ****p < 0.00001). Importantly, the ID-associated variant harboring NAV3 mRNAs could not rescue the normal developmental class of zebrafish, supporting their pathogenic nature. Error bars in (a, b) represent standard error of the mean.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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