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Fig. 6 NAV3-WT mRNA injections rescued the phenotype in morphants as compared to variants harboring mRNAs. a Bar graph representation of morphant morphological phenotypes after injection of four different concentrations (250 pg, 500 pg, 750 pg, and 1000 pg) along with nav3 ATG_MO. The 500 pg concentration of NAV3-WT mRNA injection revealed the most significance rescue of normal class larvae. NS not-significant; ***p < 0.000250; and ****p < 0.00001. b Rescue experiments performed via co-injecting human NAV3WT or variants harboring mRNAs (500 pg) along with ATG_MO (8 ng) revealed significant rescue of normal developmental class in WT mRNA injected morphants compared to ATG_MO alone. The normal phenotype class in morphants from NAV3WT and the variants mRNAs co-injected were compared for statistical analysis (***p < 0.0001; ****p < 0.00001). Importantly, the ID-associated variant harboring NAV3 mRNAs could not rescue the normal developmental class of zebrafish, supporting their pathogenic nature. Error bars in (a, b) represent standard error of the mean.