FIGURE

FIGURE 4

ID

ZDB-FIG-240417-32

Publication

Liu et al., 2024 - Plexin B3 guides axons to cross the midline in vivo

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FIGURE 4

The intracellular domain of Plexin B3 is required for signaling transduction in retinal axon guidance. (A) Two morpholinos were designed to interfere with the splicing of exon 4 (E4) and exon 21 (E21) of Plexin B3 pre-mRNA. Sequencing the mis-spliced mRNA revealed that MO^E4 results in a truncated protein remaining only part of extracellular part and MO^E21 results in a truncated protein missing the majority of intracellular domains. (B) Plexin B3 MO^E21 treatment causing misprojection of retinal axons into the ipsilateral tectum (arrow), similar as the effect of MO^E4. The image is dorsal view, rostral to the top. Scale bar, 100 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO2-plxnb3
Knockdown Reagent:	MO2-plxnb3
Observed In:	optic tectum retina axon
Stage:	Day 4

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front. Cell. Neurosci.